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Although the tiny roundworm Caenorhabditis elegans has only 302 neurons in its entire nervous system, studies of this simple animal have significantly advanced our understanding of human brain function because it shares many genes and neurochemical signaling molecules with humans. Now MIT researchers have found novel C.
Solitalea koreensis gen. nov., sp. nov. and the reclassification of [Flexibacter] canadensis as Solitalea canadensis comb. nov.
Int J Syst Evol Microbiol. 2009 Jun 30;
Authors: Weon HY, Kim BY, Lee CM, Hong SB, Jeon YA, Koo BS, Kwon SW
A Gram-negative, rod-shaped, non-spore-forming bacterium, designated strain R2A36-4T, was isolated from greenhouse soil and subjected to a polyphasic taxonomic analysis. 16S rRNA gene sequence analysis revealed that the strain represented a novel member of the family Sphingobacteriaceae. Its nearest phylogenetic neighbour was the type strain of [Flexibacter] canadansis CIP 104802T (93.2 % 16S rRNA gene sequence similarity). Both strains R2A36-4T and [Flexibacter] canadansis CIP 104802T fall into a distinct cluster within the family Sphingobacteriaceae. Strain R2A36-4T contained NK-7 as the predominant quinone. Strain R2A36-4T and [Flexibacter] canadansis CIP 104802T had iso-C15:0, iso-C17:0 3-OH and summed feature 3 as the major fatty acids. R2A36-4T was distinguishable from [Flexibacter] canadansis on the basis of several physiological properties and fatty acid compositions. On the basis of phenotypic characterization and 16S rRNA gene sequence analysis, it is proposed that strain R2A36-4T should be placed to a novel species within a new genus, for which the name Solibacillus koreensis gen. nov., sp. nov. is proposed. The type strain of Solibacillus koreensis is strain R2A36-4T (=KACC 12953T =DSM 21342T). It is also proposed that [Flexibacter] canadensis be transferred to this genus as Solibacter canadensis comb. nov.
PMID: 19567567 [PubMed - as supplied by publisher]
Transfer of Actinomadura spadix Nonomura and Ohara 1971 to Actinoallomurus spadix gen. nov., comb. nov., and description of Actinoallomurus amamiensis sp. nov., Actinoallomurus caesius sp. nov., Actinoallomurus coprocola sp. nov., Actinoallomurus fulvus sp. nov., Actinoallomurus iriomotensis sp. nov., Actinoallomurus luridus sp. nov., Actinoallomurus purpureus sp. nov. and Actinoallomurus yoronensis sp. nov.
Authors: Tamura T, Ishida Y, Nozawa Y, Otogturo M, Suzuki KI
Ten actinomycete strains that form chains of spiral or looped spores were isolated from the soil and dung samples in Japan. They contained D- and L-lysine, meso-A(2)pm, D-glutamic acid, D- and L-alanine in cell wall peptidoglycan; madurose as a characteristic whole-cell sugar; MK-9(H(6)) and MK-9(H(8)) as the major isoprenoid quinones; iso-C(16:0) as the predominant cellular fatty acid; and 69-74 mol% G+C content in the genomic DNA. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolated actinomycete strains consistently formed a monophyletic cluster with Actinomadura spadix and a separate line of descent in the phylogenetic cluster of the family Thermomonosporaceae. Actinomadura spadix also contained D- and L-lysine in addition to meso-A(2)pm. These genetic and phenotypic evidences revealed that the actinomycete strains could be clearly differentiated from the other members of the family Thermomonosporaceae and that they warranted a separate genus status. We concluded that Actinomadura spadix should be assigned the status of the type species of a new genus Actinoallomurus and referred to as Actinoallomurus spadix comb. nov. (type strain, NBRC 14099(T) =ATCC 27298(T) =BCRC 13386(T) =CBS 261.72(T) =CIP 105479(T) =DSM 43459(T) =JCM 3146(T) =KCTC 9252(T) =NCIMB 11118(T) =NRRL B-16128(T)). Further, we concluded that the isolates should be assigned the status of the following 8 novel species: Actinoallomurus amamiensis, Actinoallomurus avellaneus, Actinoallomurus bubalinus, Actinoallomurus coprocola, Actinoallomurus ferrogrisea, Actinoallomurus iriomotensis, Actinoallomurus purpurea, and Actinoallomurus yoroensis [type strains, TT00-28(T) (=NBRC 103682(T)), TT99-66(T) (=NBRC 103680(T)), TT02-15(T) (=NBRC 103683(T)), TT04-09(T) (=NBRC 103688(T)), A3015(T) (=NBRC 103678(T)), TT02-47(T) (=NBRC 103685(T)), TTN02-30(T) (=NBRC 103687(T)) and TTN02-22(T) (=NBRC 103686(T))].
PMID: 19567582 [PubMed - as supplied by publisher]
Proposal for the reclassification of the members of the genus Tetrathiobacter Ghosh et al. 2005 to the genus Advenella Coenye et al. 2005.
Authors: Gibello A, Vela AI, Martín M, Barra-Caracciolo A, Grenni P, Fernández-Garayzábal JF
The taxonomic position of the genera Advenella and Tetrathiobacter was examined. The 16S rRNA gene sequence analysis revealed that both genera are closely related representing a monophyletic cluster with high sequence similarity (98.1%- 99.7%) within the family Alcaligenaceae. The phenotypic characteristics of the type strains of Advenella incenata, Tetrathiobacter kashmirensis and Tetrathiobacter mimigardefordensis were re-examined using the API 20NE, API ZYM and API 50CH systems. Phylogenetic data together with similarities in phenotypic characteristics, G+C content and cellular acid composition suggest that they should be classified in the same genus. On the basis of the data presented, both species of the genus Tetrathiobacter should be transferred to the genus Advenella, since this genus has nomenclatural priority. Therefore, T. kashmirensis and T. mimigardefordensis should be transferred to the genus Advenella as Advenella kashmirensis comb. nov. and Advenella mimigardefordensis comb. nov.
PMID: 19567588 [PubMed - as supplied by publisher]
Lactobacillus oeni sp. nov., from wine.
Authors: Mañes-Lázaro R, Ferrer S, Rosselló-Mora R, Pardo I
Ten Lactobacillus strains, previously isolated from different Bobal grape wines from the Utiel-Requena Origin Denomination of Spain, were phylogenetically, genotypically and phenotypically characterized. The 16S rRNA genes of these strains were sequenced and phylogenetic analysis showed that they form a tight phylogenetic clade closely related to reference strains Lactobacillus satsumensis, L. uvarum and L. mali. DNA-DNA hybridization results confirmed the separate status of the strains at species level. Genotypically, strains could be differentiated from their closest neighbours by 16S-ARDRA restriction profiles and RAPD patterns. Strains were Gram-positive, facultative anaerobic rods that did not exhibit catalase activity. Phenotypically they could be distinguished from their closest relatives by several traits such as growth at pH 3.3 and by their inability to ferment saccharose, amygdalin and arbutin, and to hydrolyse aesculin. The name Lactobacillus oeni sp. nov. is proposed, with strain 59bT (= CECT 7334T= DSM 19972T) as the type strain.
PMID: 19567555 [PubMed - as supplied by publisher]
Paracoccus saliphilus sp. nov., a halophilic bacterium isolated from a saline soil.
Authors: Wang Y, Tang SK, Lou K, Mao PH, Jin X, Jiang CL, Xu LH, Li WJ
A Gram-negative, cocci to short rod shaped, non-spore-forming, moderately halophilic bacterium, designated YIM 90738T, was isolated from a salt lake in Xinjiang province, north-west China and subjected to a polyphasic taxonomy. The strain was non-motile, grew at pH 6.0-8.0 (optimal growth at pH 7.0), 10-55 degrees C (optimal growth at 37 degrees C) and at salinities of 1-15 % (w/v, optimal growth at 8%) NaCl. Ubiquinone-10 was detected as the major respiratory quinone. Cellular fatty acid profile revealed C18:1omega7c (80.4 %) as the major component, similar to that of members of the genus Paracoccus. The nearest phylogenetic neighbour was Paracoccus homiensis, as determined by 16S rRNA sequence analysis (97.5 % similarity), the DNA-DNA reassociation value obtained for Paracoccus homiensis DSM 17862T and YIM 90738T was 51.5 %. The G+C content of the genomic DNA was 60.3 mol%. On the basis of phenotypic, phylogenetic data and genotypic distinctiveness, strain YIM 90738T (=CCTCC AB 206074T =KCTC 22163T) is proposed as the type strain of a novel species of the genus Paracoccus, Paracoccus saliphilus sp. nov.
PMID: 19567556 [PubMed - as supplied by publisher]
Dokdonella soli sp. nov., a gammaproteobacterium isolated from soil.
Authors: Yoo SH, Weon HY, Anandham R, Kim BY, Hong SB, Jeon YA, Koo BS, Kwon SW
An aerobic, Gram-negative, yellow-colored, rod-shaped bacterial strain, KIS28-6T, was isolated from soil. The phylogenetic analysis revealed that strain KIS28-6T was a member of the genus Dokdonella, showing 16S rRNA gene sequence similarities of 98.2 and 96.9%, to Dokdonella fugitiva CIP 108692T and Dokdonella koreensis DSM 17203T, respectively. The strain KIS28-6T showed DNA-DNA hybridization values of 38 and 32% with Dokdonella fugitiva CIP 108692T and Dokdonella koreensis DSM 17203T, respectively. The major fatty acids (>10%) were iso-C17:1omega9c (35.7%) and iso-C17:0 (26.9%) and iso-C15:0 (11.7%), and the major respiratory quinone was ubiquinone 8. The DNA G+C content was 73.0 mol%. Based on the results of polyphasic taxonomic studies, we concluded that strain KIS28-6T should be classified in the genus Dokdonella, and we propose the name Dokdonella soli sp. nov. for this novel species. The type strain is KIS28-6T (= KACC 12741T = JCM 15421T).
PMID: 19567557 [PubMed - as supplied by publisher]
Description of Bacillus canaveralius sp. nov., an alkali-tolerant bacterium isolated from a spacecraft assembly facility.
Authors: Newcombe D, Dekas A, Mayilraj S, Venkateswaran K
Two Gram-positive, rod-shaped, alkalo-tolerant (pH 10.5), spore-forming bacteria (KSC-SF-8bT and KSC-SF-10a) were isolated from surfaces within the Payload Hazardous Servicing Facility (PHSF) where robotic spacecraft are assembled and tested before launch. Based on 16S rRNA gene sequence similarity, these strains were shown to belong to the family Bacillaceae and related to the genus Bacillus. The highest 16S rRNA gene sequence similarity was ~97.5% between the novel strains and the H2S producing B. selenatarsenatis. Several phenotypic characteristics such as growth at 10% NaCl, assimilation of D-melibiose and lactose were useful in the discrimination of this novel species from the closely related alkalo-tolerant (pH 10.5) species, B. firmus and B. selenatarsenatis. The results of DNA-DNA hybridization studies revealed that KSC-SF-8bT maintained less than 45% reassociation values with its closest genotypic neighbors. The combination of unique phenotypic and genotypic characteristics allowed the differentiation of these alkalo- and halo-tolerant spore-forming strains from related Bacillus species, for which the name Bacillus canaveralius sp. nov. is proposed. The type strain of this novel species is KSC-SF-8bT (=ATCC BAA-1493T =MTCC 8908T).
PMID: 19567559 [PubMed - as supplied by publisher]
Bifidobacterium bombi sp. nov., from the bumblebee digestive tract.
Authors: Killer J, Kopecny J, Mrázek J, Rada V, Benada O, Koppová I, Havlík J, Straka J
Strains of a novel Gram-positive, anaerobic, non-spore-forming, lactate- and acetate-producing bacterial species were isolated from the digestive tract of different bumblebees (Bombus lucorum, Bombus pascuorum and Bombus lapidarius). All selected isolates produced fructose-6-phosphate phosphoketolase activity. Cells of strain BluCI/TPT were Gram-positive with occasional bifurcation and irregular constrictions. The isolate was strictly anaerobic. The bacterium utilized a wide range of carbohydrates. Glucose was fermented to acetate and lactate. The DNA base composition was 47.2 mol% G+C. The complete 16S rRNA and partial HSP60 gene sequence were obtained and phylogenetic relationships were determined. The type strain BluCI/TPT and related isolates were located in the actinobacterial cluster and were closely related to the genera Bifidobacterium, Scardovia, Aeriscardovia and Parascardovia. The name proposed for this bacterium is Bifidobacterium bombi sp. nov.; the type strain is BluCI/TPT (=DSM 19703T =ATCC BAA-1567T).
PMID: 19567560 [PubMed - as supplied by publisher]
Emended description of the genus Polynucleobacter and the species Polynucleobacter necessarius and proposal of two subspecies, P. necessarius subsp. necessarius subsp. nov. and P. necessarius subsp. asymbioticus subsp. nov.
Authors: Hahn MW, Lang E, Brandt U, Wu QL, Scheuerl T
Heckmann and Schmidt described the genus Polynucleobacter for bacterial endosymbionts of freshwater ciliates affiliated with the genus Euplotes, and the species Polynucleobacter necessarius for obligate endosymbionts living in the cytoplasm of E. aediculatus. Pure cultures of the type strain could never be established due to the obligate nature of the symbiotic relationship between the endosymbionts and their hosts. Therefore, P. necessarius is one of a few validly described bacterial species lacking a deposited pure culture. Meanwhile, it was demonstrated that the endosymbionts used for description of the type of P. necessarius are closely related to obligately free-living strains. Similarity values of the 16S rRNA sequence obtained from the endosymbionts in the ciliate culture and free-living isolates in the range of 99.1 to 99.4 % indicate that these organisms belong to the same species. Here, we emend the description of P. necessarius by characterization of free-living strains maintained as pure cultures. The species P. necessarius is characterized by low G+C contents of the DNA (44-46 mol%), small genome sizes (1.5-2.5 Mbp), and a lack of motility. Due to distinct differences in lifestyle and genome size of P. necessarius strains, we propose to establish two subspecies, P. necessarius subsp. necessarius subsp. nov. [with a type, which is a description based on endosymbionts in the culture 'stock 15' of the ciliate E. aediculatus (ATCC 30859)], and P. necessarius subsp. asymbioticus subsp. nov. [with the type strain QLW-P1DMWA-1T (=DSM 18221T = CIP 109841T], for the obligate endosymbionts of E. aediculatus and E. harpa and obligately free-living strains, respectively.
PMID: 19567561 [PubMed - as supplied by publisher]
Corynebacterium timonense and Corynebacterium massiliense sp. nov., isolated from human blood and human articular hip liquid.
Authors: Merhej V, Falsen E, Raoult D, Roux V
Gram-positive, facultatively anaerobic, rod-shaped organisms were isolated from the blood of a patient suffering from endocarditis (strain 5401744T) and from the hip joint liquid of a patient with infected orthopedic prosthesis (strain 5402485T). These strains were characterized by a polyphasic taxonomic approach. Based on cellular morphology and biochemical criteria the two isolates were tentatively assigned to the genus Corynebacterium, although they did not correspond to any recognized species. The predominant fatty acids were a mix of C18:2omega6,9c and C18:0 anteiso (32.1%), C16:0 (26.3%), and C18:1omega9c (22.5%) for strain 5402485T and C18:1omega9c (36.4%), C17:1omega9c (27.1%) and C16:0 (10.9%) for strain 5401744T. Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that strain 5401744T was closely related to C. auris, C. capitovis, C. lipophiloflavum and C. mycetoides (97.0 %, 96.6%, 96.5 % and 96.3% of similarity, respectively) and strain 5402485T was closely related to C. macginleyi and C. accolens, C. tuberculostearicum, C. confusum, C. mastitidis and C. renale (95.6%, 95.3%, 95.3%, 94.5%, 94.0% and 93.5% respectively). On the basis of phenotypic data and phylogenetic inference, we propose that these isolates represent two novel species Corynebacterium timonense sp. nov. (type strain 5401744T =CSUR P20T =CIP 109424T =CCUG 53856T) and Corynebacterium massiliense sp. nov (type strain 5402485T =CSUR P19T =CIP 109423T =CCUG 53857T).
PMID: 19567562 [PubMed - as supplied by publisher]
Streptomyces hypolithicus sp. nov., isolated from an Antarctic hypolith community.
Authors: Le Roes-Hill M, Rohland J, Meyers PR, Cowan DA, Burton SG
As part of an enzyme-screening program, the actinomycete strain HSM#10(T) was isolated from a sample collected from the base of a hypolithon, Miers Valley, Eastern Antarctica. The isolate produced branching vegetative mycelia characteristic of actinomycetes. The chemotaxonomic characteristics of the strain corresponded with those of the members of the family Streptomycetaceae Waksman and Henrici 1943 [emend. Rainey et al. 1997 (in Stackebrandt et al., 1997), emend. Kim et al. 2003]. Furthermore, 16S rRNA gene phylogenetic analysis showed that the strain is closely related to members of the genus Streptomyces, which supports the classification of this strain within the family Streptomycetaceae. Phenotypic and phylogenetic results allowed for the differentiation of strain HSM#10(T) from known streptomycetes. DNA-DNA hybridization further differentiated strain HSM#10(T) from its nearest phylogenetic neighbours, Streptomyces chryseus DSM 40420(T) (53.55+/-3.15% DNA relatedness), Streptomyces helvaticus DSM 40431(T) (38.75+/-2.75% DNA relatedness), Streptomyces flavidovirens DSM 40150(T) (30.7 +/- 2.90% DNA relatedness) and Streptomyces albidochromogenes DSM 41800(T) (33.9+/-0.10% DNA relatedness). The name Streptomyces hypolithicus, with the type strain HSM#10(T) (=DSM 41950(T) =NRRL B-24669(T)), is proposed.
PMID: 19567563 [PubMed - as supplied by publisher]
Microvirga guangxiensis sp. nov., a novel alphaproteobacterium from soil, and emended description of the genus Microvirga.
Authors: Zhang J, Song F, Xin YH, Zhang J, Fang C
A Gram-negative bacterium designated strain 25BT, was isolated from a soil sample from a rice field in Guangxi Province, China, and its taxonomic position was investigated by using a polyphasic approach. The cells were rod-shaped, non-spore-forming, non-motile and strictly aerobic. Strain 25BT grew optimally at 37 degrees C and pH 7.0. The predominant fatty acids of this soil isolate were C18:1omega7c, C19:0 cyclo omega8c and C16:0. Phylogenetic analysis based on almost complete 16S rRNA gene sequences showed that strain 25BT formed a monophyletic clade with the type strain of Microvirga subterranea; the two organisms share 97.2% 16S rRNA gene similarity. However, the two strains shared a low DNA-DNA relatedness value. Strain 25BT also had a profile of phenotypic properties that readily distinguished it from its only close neighbour, Microvirga subterranea DSM 14364T. On the basis of the combination of genotypic and phenotypic data, the organism represents a novel species of the genus Microvirga, for which the name Microvirga guangxiensis sp. nov. is proposed. The type strain is 25BT (=CGMCC 1.7666T =JCM 15710T).
PMID: 19567564 [PubMed - as supplied by publisher]
Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella.
Authors: Tang SK, Wang Y, Chen Y, Lou K, Cao LL, Xu LH, Li WJ
A Gram-positive, non-motile, white-pigmented, short rod actinobactium, designated YIM 90734T, was isolated from a saline soil sample collected from Ganjiahu Suosuo Forest National Nature Reserve in Xinjiang province, north-west China, and its taxonomic position was investigated by using a polyphasic approach. Strain YIM 90734T grew optimally at 28-37 degrees C, pH 6.0-8.0 and 5 % (w/v) NaCl. The peptidoglycan type was A4alpha, L-Lys-L-Ala-L-Glu and tyvelose and mannose as major cell-wall sugars. The predominant menaquinones were MK-10 and MK-9. Major cellular fatty acids (>10 % of total) was anteiso-C15:0 and anteiso-C17:0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phospholipid and two unknown glycolipid. The DNA G+C content was 70.3 mol %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 90734T belongs to the genus Zhihengliuella. Similarity value, between the 16S rRNA gene sequence of strain YIM 90734T and the only type strain of recognized Zhihengliuella species, Zhihengliuella halotolerans, was 97.7 %. Meanwhile, their DNA-DNA hybridization with each other showed a relatedness of 41.4 %. DNA-DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain YIM 90734T could be differentiated from Zhihengliuella halotolerans. On the basis of the data presented, strain YIM 90734T is considered to represent a novel species of the genus Zhihengliuella, for which the name Zhihengliuella alba sp. nov. is proposed. The type strain is YIM 90734T (=KCTC 19375T =DSM 21143T). Furthermore, the description of the genus Zhihengliuella is also required to be emended.
PMID: 19567565 [PubMed - as supplied by publisher]
Roseibaca ekhonensis gen. nov., sp. nov., an alkalitolerant and aerobic bacteriochlorophyll a-producing alphaproteobacterium from hypersaline Ekho Lake.
Authors: Labrenz M, Lawson PA, Tindall BJ, Hirsch P
A Gram-negative, aerobic rod was isolated from 6 m depths of the hypersaline, heliothermal and meromictic Ekho Lake (East Antarctica). The strain is oxidase- and weakly catalase-positive and metabolises a variety of carboxylic acids, alcohols, sugars or lipids. Cells have an absolute requirement for artificial seawater (ASW) or NaCl. Optimal growth occurs at 16 degrees C and at pH values ranging from 7.0 to 9.5. A large in vivo absorption band at 865-866 nm indicated production of bacteriochlorophyll (bchl) a. The predominant fatty acid of this organism is 18:1omega7c, with 3-OH 14:1, 16:1omega9c, 16:0 and 18:1omega9c present in lower amounts. 16:0 and 18:1omega9c were probably amide-linked. The main polar lipids are diphosphatidylglycerol, phospatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. Ubiquinone 10 is produced. Cell wall diamino acid is m-diaminopimelic acid. The DNA G+C content of this strain is 61 mol%. 16S rRNA gene sequence comparisons show the isolate is with approximately 96% similarity phylogenetically most closely related to alkaliphilic Rhodobaca and Roseinatronobacter species. The organism has no particular relationship to any other cultivated members within the alpha-Proteobacteria. Distinct morphological, physiological and genotypic differences to these previously described taxa support the description of a new genus and species, Roseibaca ekhonensis gen. nov. sp. nov. The type strain is EL-50T (= DSM 11469T = CECT 7235T).
PMID: 19567566 [PubMed - as supplied by publisher]
Gordonia kroppenstedtii sp. nov., a phenol-degrading actinomycete isolated from a polluted stream.
Authors: Kim KK, Lee KC, Klenk HP, Oh HM, Lee JS
A phenol-degrading actinomycete, strain NP8-5T, was isolated from a polluted stream in Gumi, Korea. The strain was aerobic, Gram-positive, non-motile and non-spore-forming, displayed a rod-coccus growth cycle, exhibited white opaque colonies on complex media, and showed chemotaxonomic markers that were consistent with the classification in the genus Gordonia, i.e. meso-diaminopimelic acid, arabinose and galactose in whole-cell hydrolysates, N-glycolylmuramic acid in the peptidoglycan wall, unbranched saturated and monounsaturated fatty acids plus tuberculostearic acid (TBSA), mycolic acids that comprised 56-60 carbon atoms, MK-9(H2) as the predominant menaquinone and a DNA G+C content of 68.5 mol%. Phylogenetic analysis, based on 16S rRNA gene sequencing, also showed that strain NP8-5T belonged to the genus Gordonia, which shared the highest levels of sequence similarity with Gordonia araii IFM 10211T, Gordonia hydrophobica DSM 44015T and Gordonia sinesedis NCIMB 13802T (96.4, 96.0 and 95.9 %, respectively) and formed a separate lineage with G. sinesedis. Combined phylogenetic and phenotypic data supported that strain NP8-5T represents a novel species of the genus Gordonia, for which the name Gordonia kroppenstedtii sp. nov. is proposed; the type strain is NP8-5T (=KCTC 19360T =DSM 45133T).
PMID: 19567568 [PubMed - as supplied by publisher]
Aliagarivorans marinus gen. nov., sp. nov. and Aliagarivorans taiwanensis sp. nov., facultatively anaerobic marine bacteria capable of agar degradation.
Authors: Jean WD, Huang SP, Liu TY, Chen JS, Shieh WY
Two agarolytic strains of Gram-negative, heterotrophic, facultatively anaerobic, marine bacteria, designated AAM1(T) and AAT1(T) , were isolated from seawater samples collected in the shallow coastal region of An-Ping Harbour, Tainan, Taiwan. Cells grown in broth cultures were straight rods, motile by means of a single polar flagellum. The two isolates required NaCl for growth and grew optimally at about 25-30 degrees C, 2-4 % NaCl and pH 8. They grew aerobically and could achieve anaerobic growth by fermenting D-glucose or other sugars. Major isoprenoid quinone was Q-8 (79.8-92.0 %). Major cellular fatty acids were C16:1omega7c and/or iso-C15:0 2-OH (26.4-35.6 %), C18:1omega7c (27.1-31.4 %) and C16:0 (14.8-16.3 %). AAM1(T) and AAT1(T) had DNA G+C contents of 52.9 and 52.4 mol%, respectively. The two strains had a 16S rRNA gene sequence similarity of 98.6 % and shared 84.9-92.4 % sequence similarity with the closest phylogenetic species, Agarivorans albus (91.2-92.4 %), Alteromonas spp. (84.9-87.1 %), Aestuariibacter spp. (86.0-87.0), Bowmanella denitrificans (86.1-86.7 %), Glaciecola spp. (85.0-87.9 %) and Salinimonas chungwhensis (85.9-86.1 %). Despite the high sequence similarity, AAM1(T)and AAT1(T) had a DNA-DNA relatedness value of only 4.5 %. The polyphasic data obtained in this study revealed that the two agarolytic isolates could be classified as representatives of two novel species in a new genus, Aliagarivorans gen. nov., with Aliagarivorans marinus sp. nov. (type strain AAM1(T) = BCRC 17888(T) = JCM 15522(T) ) as the type species and Aliagarivorans taiwanensis sp. nov. (type strain AAT1(T) = BCRC 17889(T) = JCM 15537(T) ) as a second species.
PMID: 19567569 [PubMed - as supplied by publisher]
Brevibacterium massiliense sp. nov., isolated from a human ankle discharge.
Authors: Roux V, Raoult D
Gram-positive, non spore-forming rods were isolated from a human ankle discharge (strain 5401308T). Based on cellular morphology and the results of biochemical testings, this strain was tentatively identified as an undescribed species of the genus Brevibacterium. The major fatty acids were C15:0 anteiso (45.3%), C17:0 anteiso (19.2%) and C15:0 iso (18.3%). Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that the bacterium was closely related to B. mcbrellneri (96.3% similarity) and B. paucivorans (95.8%). On the basis of phenotypic data and phylogenetic inference, it is proposed that this strain should be designated Brevibacterium massiliense sp. nov., the type strain is strain 5401308T (=CSUR P26T =CIP 109422T =CCUG 53855T).
PMID: 19567570 [PubMed - as supplied by publisher]
